Effects of glucose on insulin release and 86Rb permeability in cultured neonatal and adult rat islets |
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Authors: | A C Boschero D Tombaccini I Atwater |
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Institution: | Laboratory of Cell Biology and Genetics, NIDDK, Bethesda, MD 20892. |
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Abstract: | Glucose-induced insulin release and modifications in 86Rb outflow were studied in cultured neonatal and adult rat islets. The dose-response curve for neonatal islets was steeper than for adult islets and the maximal response was clearly shifted towards lower glucose concentrations. In neonatal islets, glucose-induced insulin release was inhibited by the Ca2+-channel blocker, nifedipine. In the absence of glucose, the 86Rb outflow from neonatal islets was lower than from adult islets. Also, the glucose-induced reduction in 86Rb outflow was less pronounced in neonatal islets. Altered K+ permeability in the B-cell membrane could explain the change in glucose sensitivity of neonatal islets. |
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