Relationships among Leymus species assessed by RAPD markers |
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Authors: | R. W. Yang Y. H. Zhou C. B. Ding Y. L. Zheng L. Zhang |
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Affiliation: | (1) College of Biology and Science, Sichuan Agricultural University, Yaan, 625014 Sichuan, P.R. China;(2) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, 611830 Sichuan, P.R. China |
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Abstract: | The DNA genetic diversity of 40 accessions of genus Leymus was analyzed by random amplified polymorphic DNA (RAPD) markers. A total of 352 products were amplified by 34 10-mer arbitrary primers, among which 337 products (95.74 %) were found to be polymorphic. 5–14 polymorphic bands were amplified by each polymorphic primer, with an average of 9.91 bands. The data of 352 RAPD bands were used to generate Jaccard’s similarity coefficients and to construct a dendrogram by means of UPGMA. Great genetic diversity in genus Leymus was observed, the genetic diversity among the different species more abundant than that of the different accessions, and the different accessions in a species or the species from the same areas were clustered together. |
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Keywords: | KeywordHeading" >Additional cluster analysis genetic diversity molecular marker polymerase chain reaction similarity coefficients |
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