Oligonucleotides with pyrene fluorophore at the sugar fragment: synthesis and properties in binding to complementary polynucleotide |
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| Authors: | K Yamana T Gokota Y Ohashi H Ozaki M Kitamura H Nakano O Sangen T Shimidzu |
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| Affiliation: | Department of Applied Chemistry, Himeji Institute of Technology, Japan. |
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| Abstract: | Oligonucleotides with 1-pyrenylmethyl substituent at the designated sugar residue were synthesized by using 5'-dimethoxytrityl 2'-(1-pyrenylmethyl)uridine 3'-phosphorobisdiethylamidite 1. It was shown that the pyrene-oligonucleotides have enhanced affinity in binding to the complementary polynucleotide sequence. The fluorescence yield and life time of the pyrene-oligonucleotide was drastically enhanced when bound by the complementary polynucleotide. |
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