Involvement of a Ca2+-calmodulin interaction in the yeast-mycelial (Y-M) transition of Candida albicans |
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Authors: | F. T. Sabie G. M. Gadd |
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Affiliation: | (1) Department of Biological Sciences, University of Dundee, DD1 4HN Dundee, Scotland, UK |
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Abstract: | A yeast-mycelium (Y-M) transition of Candida albicans (3153A) was induced by 1.5 mM CaCl2 · 2H2O in defined liquid medium, pH 7, at 25 °C. Germ tube formation was detected after approximately 8 h and peaks of maximum germination occurred at approximately 20 h in all experimental treatments. Non-toxic concentrations of the calmodulin inhibitor R24571 almost completely suppressed germ tube formation whereas trifluoperazine (TFP) and the Ca2+ ionophore A23187 were only about half as effective. Further Ca2+ addition failed to reverse the inhibitory effect of R24571 and induced only about 10% of the cells inhibited by TFP or A23187 to germinate. |
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Keywords: | Candida albicans dimorphism yeast-mycelium transition calcium calmodulin |
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