Tight junctions of dissociated and reaggregated embryonic lung cells |
| |
| Authors: | A Talmon Y Ben-Shaul |
| |
| Institution: | 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, China;2. Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China;3. Department of Animal Resource and Science, Dankook University, Anseodong, South Korea;1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China;2. Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan, Hubei 430070, China;3. College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China;4. Wuhan HUAYU XINMEI Mushroom industry Company Limited, Wuhan 430070, China |
| |
| Abstract: | Treatment of embryonic lung tissue with trypsin resulted in clustering of intramembrane particles (IMP) and gradual disassembly of tight junctions. In dissociated single cells kept in trypsin-free medium, IMP are randomly distributed but degradation of tight junctions continue. Vesicles containing tight junction elements were observed within the cytoplasm. It is therefore assumed that tight junctions may be degraded in two ways: breakdown of elements to IMP, and endocytosis. In cells reaggregated by rotation tight junctions reassembled only in hystotypic aggregates. Cycloheximide which interferes with histotypic reaggregation prevents the reassembly of tight junctions. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
