Reversible change in the fibroblast lysosomal enzyme dipeptidyl aminopeptidase-1 (cathepsin c) related to the commercial source of fetal bovine serum in the culture medium |
| |
Authors: | Michael J. Doughty Michael H. Davis Eric Gruenstein |
| |
Affiliation: | (1) University of Cincinnati Medical Center, 231 Bethesda Avenue, 45267 Cincinnati, Ohio;(2) Present address: School of Optometry, University of Waterloo, N2L 391 Waterloo, Ontario, Canada |
| |
Abstract: | Summary The commercial source of fetal bovine serum used to supplement the growth medium of human skin fibroblasts alters the activity of the lysosomal enzyme dipeptidyl aminopeptidase-1 (DAP-1). Cells grown with one serum were found to have a threefold higher level of DAP-1 than those grown with serum from another source (P<0.001). The effect on DAP-1 activity was specific inasmuch as no differences were found in the activities of a variety of other lysosomal and nonlysosomal hydrolases: DAP-II, DAP-III, DAP-IV, β-glucosidase, β-glucuronidase, andN-acetyl-β-galactosaminidase. The effect is reversible and is observed over a wide range of cell population doublings. Cell growth kinetics were not significantly different with the different sera. This work was supported in part by grants from the National Institutes of Health, Bethesda, MD (NS 16287). |
| |
Keywords: | human fibroblasts lysosomal enzymes cathepsin C dipeptidyl aminopeptidase-1 fetal bovine serum |
本文献已被 SpringerLink 等数据库收录! |
|