QTL, additive and epistatic effects for SCN resistance in PI 437654 |
| |
Authors: | Xiaolei Wu Sean Blake David A Sleper J Grover Shannon Perry Cregan Henry T Nguyen |
| |
Institution: | (1) Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri-Columbia, Columbia, MO 65211, USA;(2) University of Missouri, Delta Center, P.O. Box 160, Portageville, MO 63873, USA;(3) Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA |
| |
Abstract: | PI 437654 is a unique accession because of its resistance to nearly all HG types (races) of soybean cyst nematode (Heterodera glycines Ichinohe; SCN). Objectives of this study were to confirm and refine the locations and gene action associated with SCN resistance
previously discovered in PI 437654, and to identify new QTLs that may have been missed because of low coverage with genetic
markers used in previous studies. Using 205 F7:9 RILs and 276 SSR and AFLP molecular markers covering 2,406.5 cM of 20 linkage groups (LGs), we confirmed and refined the
locations of major SCN resistance QTLs on LG-A2, -B1, and -G previously identified in PI 437654 or other resistant sources.
We found that these major QTLs have epistatic effects among them or with other loci for SCN resistance. We also detected some
new QTLs with additive or epistatic effects for SCN resistance to different HG types (races) on all LGs except LGs-B2 and
-D1b. The QTL on LG-G was associated with resistance to HG types 2.5.7, 1.2.5.7, 0, and 2.7 (races 1, 2, 3, and 5), and it
contributed a large proportion of the additive effects. The QTL on LG-A2 was associated with resistance to HG types 2.5.7
and 0 (races 1 and 3). The QTL on LG-B1, associated with resistance to HG types 2.5.7, 0, 2.7 (races 1, 3, and 5), was the
similar QTL found in PI 90763 and PI 404198B. In addition to QTL on LGs-A2, -B1 and -G, a novel additive QTL associated with
SCN resistance to HG types 0, 2.7, and 1.3.5.6.7 (race 3, 5, and 14) was identified on LG-I flanked by Sat_299 and Sat_189.
Several minor QTLs on LGs-C1, D1a, H, and K were also found to be associated with SCN resistance. Confirmation of the new
resistance QTL is underway by evaluating another RIL population with a different genetic background. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|