Purification of glutathione S‐transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)‐2‐(4‐((E)‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1H‐4,7‐methanoisoindole‐1,3(2H)‐dione derivatives on the enzyme activity |
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Authors: | Yusuf Temel Umit M. Koçyigit M. Şerif Taysı Faik Gökalp Meliha Burcu Gürdere Yakup Budak Mustafa Ceylan İlhami Gülçin Mehmet Çiftci |
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Affiliation: | 1. Solhan Vocational School of Health Services, Bingol University, Bingol 12000, Turkey;2. Vocational School of Health Services, Cumhuriyet University, Sivas 58140, Turkey;3. Department of Chemistry, Faculty of Arts and Sciences, Bingol University, Bingol 12000, Turkey;4. Department of Mathematics and Science Education, Faculty of Education, K?r?kkale University, K?r?kkale 71450, Turkey;5. Department of Chemistry, Faculty of Arts and Sciences, Gaziosmanpasa University, Tokat 60250, Turkey;6. Department of Chemistry, Faculty of Science, Atatürk University, Erzurum 25240, Turkey |
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Abstract: | The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S‐transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88‐fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS‐PAGE method and showed a single band. In addition, inhibition effects of (3aR,4S,7R,7aS)‐2‐(4‐((E)‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1H‐4,7methanoisoindole‐1,3(2H)‐dion derivatives ( 1a–g ) were investigated on the enzyme activity. The inhibition parameters (IC50 and Ki values) were calculated for these compounds. IC50 values of these derivatives ( 1a–e ) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. Ki values of these derivatives ( 1a–e ) were calculated in the range of 3.04 ± 0.50 to 131.50 ± 32.50 μM. However, for f and g compounds, the inhibition effects on the enzyme were not found. |
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Keywords: | Quail liver GST inhibition methanoisoindole enzyme purification |
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