The effect of astaxanthin and cadmium on rat erythrocyte G6PD, 6PGD,GR, and TrxR enzymes activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro |
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| Authors: | Mahire Bayramo?lu Akkoyun A ?ükrü Bengü Yusuf Temel H Turan Akkoyun Suat Ekin Mehmet Ciftci |
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| Institution: | 1. Faculty of Veterinary, Siirt University, Siirt, Turkey;2. Health Services Vocational School, Bingol University, Bingol, Turkey;3. Solhan Health Services Vocational School, Bingol University, Bingol, Turkey;4. Department of Chemistry, Faculty of Sciences, Yüzüncü Y?l University, Van, Turkey;5. Department of Chemistry, Faculty of Arts and Sciences, Bingol University, Bingol, Turkey |
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| Abstract: | In this study, the effects of astaxanthin (AST) that belongs to carotenoid family and cadmium (Cd), which is an important heavy metal, on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzyme activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro were studied. In in vitro studies, 6PGD enzyme was purified from rat erythrocytes with 2′,5′‐ADP Sepharose4B affinity chromatography. Results showed inhibition of enzyme by Cd at IC50; 346.5 μM value and increase of 6PGD enzyme activity by AST. In vivo studies showed an increase in G6PD, 6PGD, and GR enzyme activities (P ? 0.05) and no chance in TrxR enzyme activity by AST. Cd ion inhibited G6PD, 6PGD, and GR enzyme activities (P ? 0.05) and also decreased TrxR enzyme activity (P ? 0.05). AST + Cd group G6PD enzyme activity was statistically low compared with control group (P ? 0.05). 6PGD and TrxR enzyme activities decreased without statistical significance (P ? 0.05); however, GR enzyme activity increased statistically significantly (P ? 0.05). |
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| Keywords: | 6PGD astaxanthin cadmium G6PD GR TrxR |
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