| 海巴戟果水溶性多糖的分离纯化及清除自由基活性 |
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| 引用本文： | 刘海青,刘银才,胡文婷.海巴戟果水溶性多糖的分离纯化及清除自由基活性[J].生物加工过程,2008,6(3):44-47. |
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| 作者姓名： | 刘海青 刘银才 胡文婷 |
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| 作者单位： | 海南大学,海洋学院,海口,570228 |
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| 基金项目： | 海南大学校科研和教改项目 |
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| 摘 要： | 采用热水提取乙醇沉淀获得海巴戟水溶性粗多糖,经DEAE-Sephadex A-50分离纯化得海巴戟果水溶性多糖（MOCI）。经纸层析、醋酸纤维薄膜电泳和SepharoseCl-4B柱层析纯度鉴定表明,MOCI为相对分子质量均一的纯多糖。抗衰老活性研究结果表明,MOCI能有效清除.OH和O2^-.自由基。
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| 关 键 词： | 海巴戟 多糖 分离纯化 清除 自由基 |
| 文章编号： | 1672-3678(2008)03-0044-04 |
| 修稿时间： | 2007年6月6日 |
Isolation water soluble polysaccharide from Morinda cirtifolia and its scavenging free radical actvity |
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| LIU Hai-qing,LIU Yin-cai,HU Wen-ting.Isolation water soluble polysaccharide from Morinda cirtifolia and its scavenging free radical actvity[J].Chinese Journal of Bioprocess Engineering,2008,6(3):44-47. |
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| Authors: | LIU Hai-qing LIU Yin-cai HU Wen-ting |
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| Institution: | (College of Oceanography, Hainan University, Haikou 570228, China) |
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| Abstract: | The crude polysaccharide extracted from the fruits of Morinda citrifolia with hot water was deproteinized by the combination of enzymatic and Sevag methods. The polysaccharide MOCI was obtained by DEAE-Sephadex A-50. The purity identification by gel chromatography showed that MOCI was an uniform polysaccharide. Free radical scavenging experiment showed that MOCI was effective in scavenging superoxide radical and hydroxyl radical. |
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| Keywords: | Morinda citrifolia polysaccharide isolation scavenging free radical |
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