Specific estrogen binding proteins in human cervix. |
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| Authors: | B M Sanborn B Held H S Kuo |
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| Affiliation: | 1. Programs in Reproductive Biology and Endocrinology The University of Texas Medical School at Houston, Houston, Texas 77025, U.S.A.;2. Obstetrics and Gynecology The University of Texas Medical School at Houston, Houston, Texas 77025, U.S.A.;1. Drug Product Design and Development, Medicinal Science and Technology, GlaxoSmithKline, Stevenage, Herts, SG1 2NY, UK;2. In-vitro and In-vivo Translation, Research, GlaxoSmithKline, Stevenage, Herts, SG1 2NY, UK;3. In-vitro and In-vivo Translation, Research, GlaxoSmithKline, Ware, Herts, SG12 0DP, UK;4. Drug Design and Selection, Medicinal Science and Technology, GlaxoSmithKline, King of Prussia, Pennsylvania 19406;5. Drug Product Design and Development, Medicinal Science and Technology, GlaxoSmithKline, King of Prussia, Pennsylvania 19406;1. Division of Maternal-Fetal Medicine and Perinatal Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, USA;2. Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Ermita, Manila, Philippines;1. MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, Department of Medical Chemistry, Faculty of Medicine, H-6720 Dóm tér 8, Szeged, Hungary;2. Department of Physical Chemistry and Material Sciences, University of Szeged, H-6720 Aradi Vt. tere 1, Szeged, Hungary;1. Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna Street, 30-343 Kraków, Poland;2. Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688 Kraków, Poland;3. Center of Physiology, Hannover Medical School, 1 Carl-Neuberg Street, 30625 Hannover, Germany;4. Laboratory of Cell Biophysics, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warszawa, Poland;5. Adam Mickiewicz University, Faculty of Chemistry, 89b Umultowska Street, 61-614 Poznań, Poland;6. CERKO, 96/98 Zwycięstwa Avenue, 81-525 Gdynia, Poland;1. Institute of Biochemistry, Saarland University, Campus B2.2, 66123 Saarbruecken, Germany;2. Pharmaceutical Biology, Saarland University, Campus C2.2, 66123 Saarbruecken, Germany |
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| Abstract: | An estrogen binding protein has been found in all regions of the human cervix. The greatest concentration per mg protein was associated with the region including the columnar epithelium, less associated with the squamous epithelium, and an intermediate concentration in the remaining stroma. These concentrations were 5–10-fold lower than those found in the corresponding uterine tissue, but the association constants were similar (0.9–2.6 nM−1). The dissociation rate constant was found to be 0.74 × 10−6 s−1 at 0° and a sedimentation coefficient of 4.2–4.8S plus aggregates was observed. The binding was highly specific for estrogens (estradiol > estrone > estriol) but was unaffected by progesterone, testosterone, or cortisol in the nM range. Synthetic estrogens were also potent competitors (ethinyl-estradiol > estradiol > diethylstilbestrol > mestranol).Uterine estrogen binding sites were significantly higher in the proliferative phase of the menstrual cycle than in the secretory phase, expressed either per g wet weight or per mg protein (P <0.01). Cervical estrogen binding sites were significantly higher in the proliferative phase when expressed per g wet weight (P < 0.05) but not per mg protein.It is concluded that human cervix contains estrogen binding proteins with properties similar to those found in the corpus. The data collected so far suggest that levels of this binding activity may fluctuate in cervix in a manner similar to that found in endometrium. |
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