宫颈癌病变组织人乳头瘤病毒感染率的免疫细胞化学与DNA杂交的比较观察

近年来人乳头瘤病毒（ＨＰＶ）被认为与宫颈癌的发生有密切关系。该实验报道了用分子杂交技术（在严格条件下）检测了宫颈活检组织：慢性宫颈炎、宫颈上皮内瘤（ＣＩＮ）、宫颈癌和宫颈正常组织中１６型乳头瘤病毒（ＨＰＶ－１６）ＤＮＡ的阳性率，并将其与用免疫细胞化学方法（ＰＡＰ）所得结果进行了比较。结果显示１３％（２／１５）的慢性宫颈炎、１７％（２／１２）的ＣＩＮ、５６％（５１／９１）的宫颈癌含有ＨＰＶ－１６ＤＮＡ，而正常组织为阴性；ＨＰＶ属抗原仅见于３５％（６／１７）的ＣＩＮ，其他受检组织均为阴性。结果提示用ＰＡＰ法检查ＨＰＶ－１６的抗原，所得阳性率不及ＤＮＡ杂交所得阳性率高；但它作为一种简便、快速的方法用于福尔马林固定、石腊切片材料，显示ＨＰＶ属抗原，借以筛选多种不同型的ＨＰＶ对ＣＩＮ或其他组织中ＨＰＶ的增殖性感染有一定的价值。

COMPARISON OF FREQUENCY OF HPV INFECTION DETECTED BY PAP METHOD WITH THAT FOUND BY DNA HYBRIDIZATION TECHNIQUE

Recently, it has been widely recognized that human papilloma virus (HPV) infection plays an important role in the etiology of cervical cancer. The frequency of HPV--16 DVA detected in cervical biopsies with chronic cervjcitis, cervical intraepithelial neoplasia (CIN) and cervical cancer was investigated with a dot blot DNA hybridization under stringent conditions and compared with that of the detection of HPV genus--specific structural antigens by an immunocytochemical method PAP on formalin--fixed, paraffin--embeded tissues. The results showed that 2 (13%) out of 15 chronic cervicitis, 2 (17 % ) out of 12 CIN, 51 (56%) out of 91 cervical cancer were postive for HPV--16 DNA and the normal samples were negative. The antigens of HPV were only found in 6 (35%) out of 17CIN, no case of chronic cervicitis or cervical cancer revealed postive antigen staining. The recults suggested that the immunocytochemical method PAP can be used as a simple and rapid screening technique for routinely processed material to detect HPVs infection which is suspected in CIN and some other tissues.