Interaction of cultured skin cells with the polylactide matrix coved with different collagen structural isoforms

These experiments were done to optimize the surface properties of polylactide film designed for human keratinocyte cultivation. The film was covered with type 1 collagen to increase its hydrophilic properties. The method of protein application determines the consistency the matrix coating and the formation of different collagen structures on the film. Substrates modified by collagen affect the growth of cultured keratinocytes. Our data on keratinocyte spreading, morphology and cytoskeleton organization demonstrate that the best form of collagen to use to cover the polymeric surface is the fibrillar isoform.