Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol |
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| Authors: | U Finckh P A Lingenfelter D Myerson |
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| Affiliation: | Fred Hutchinson Cancer Research Center, Seattle, WA 98104. |
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| Abstract: | We report an efficient procedure to synthesize either single- or double-stranded probes labeled with biotin-11-dUTP, biotin-21-dUTP or digoxigenin-11-dUTP. To produce the single-stranded probe, only a single primer is utilized in a Taq polymerase amplification of 55 cycles. A cytomegalovirus probe is presented. This procedure allows easy production of nonradioactively labeled pure single-stranded probes of any desired length and specificity. |
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