Study on interaction between duodenase protease with dual specificity and inhibitors of bowman-birk family |
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Authors: | Gladysheva Inna P Gladysheva Popykina Natalia A Zamolodchikova Tatyana S Larionova Natalia I |
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Affiliation: | Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899 Russia. |
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Abstract: | The interaction between duodenase and inhibitors of Bowman-Birk type from soybeans (BBI) and lima beans (LBI) was investigated. Duodenase was shown to interact only with antichymotrypsin site of these inhibitors. The inhibition constants of duodenase by BBI, LBI, BBI-trypsin and LBI trypsin complexes were 4, 23, 400, 600 (n)M respectively. |
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