Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system |
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| Authors: | dos Santos Ferreira Beatriz Branco Alan Trindade de Queiroz Karlla Salim de Souza Filho Gonçalo Apolinàrio |
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| Institution: | Laboratorio de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, CEP 28015-620, Campos dos Goytacazes-RJ, Brazil. |
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| Abstract: | A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts. |
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