Purification and characterization of a new lectin from the red marine alga Hypnea musciformis |
| |
| Authors: | Nagano C S Moreno F B M B Bloch C Prates M V Calvete J J Saker-Sampaio S Farias W R L Tavares T D Nascimento K S Grangeiro T B Cavada B S Sampaio A H |
| |
| Institution: | Laboratório de Bioquímica Marinha - BioMol-Lab, Depto de Engenharia de Pesca, Universidade Federal do Ceará, Caixa Postal 6033, CEP 60451-970, Brasil. |
| |
| Abstract: | A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF/MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
