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Polymorphic CAC/T repetitive sequences in the pig genome 1 |
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| Authors: | W Coppieters A Van de Weghe A Depicker J Coppieters L Peelman A VAN Zeveren Y Bouquet |
| Affiliation: | Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43, B-4000 Liège, Belgium;Department of Animal Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, University of Ghent, Heidestraat 19, Merelbeke, B-9280, Belgium;Department of Molecular Genetics, Faculty of Sciences, University of Ghent, KL Ledeganckstraat 35, Ghent, B-9000, Belgium |
| Abstract: | Three genomic clones were isolated from a sizeselected pig DNA library by hybridization with a DNA-fingerprint probe. Analysis at the sequence level revealed that all three clones contain interrupted stretches of triplet repeats mainly composed of CAC and CAT triplets. Evaluation of the corresponding loci for polymorphism by Southern blot hybridization showed considerable length variation. For two loci the polymorphism was also demonstrated by polymerase chain reaction (PCR) amplification. The PiGMaP reference pedigree was typed for all three loci. |
| Keywords: | genetic map microsatellite minisatellite pig triplet repeat |