Transformed calli obtained by direct gene transfer into sunflower protoplasts |
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| Authors: | Anne-Laure Moyne Denis Tagu Véronique Thor Catherine Bergounioux Georges Freyssinet Pierre Gadal |
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| Affiliation: | (1) Laboratoire de Physiologie Végétale Moléculaire, Université Paris-Sud, bâtiment 430, F 91405 Orsay Cedex, France;(2) Rhône-Poulenc Agrochimie, Biologie Moléculaire et Cellulaire Végétale, 14-20 rue Pierre Baizet, BP 9163, F 69263 Lyon Cedex 09, France |
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| Abstract: | Helianthus annuus protoplasts were transformed with the plasmid pCaMVNEO (Frommet al. 1986) conferring kanamycin resistance to plant. Transformed calli were selected with a frequency of 4 calli for 106 treated protoplasts. DNA was extracted from kanamycin resistant calli. Analysis of this DNA shows the presence of the NPTII gene.Abbreviations BAP 6-benzylaminopurine - 2,4-D 2,4 dichlorophenoxyacetic acid - IAA Indole-3-acetic acid - NAA 1-naphtalenoacetic acid - NPT Neomycin phosphotransferase - PEG Polyethyleneglycol |
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| Keywords: | sunflower protoplasts direct gene transfer |
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