| 热休克反应中小鼠肾HSP70和乳酸脱氢酶同工酶表达变化的研究 |
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| 引用本文： | 魏纪东,张顺利,张新胜,张文学,徐存拴.热休克反应中小鼠肾HSP70和乳酸脱氢酶同工酶表达变化的研究[J].细胞生物学杂志,2002,24(4):242-245. |
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| 作者姓名： | 魏纪东 张顺利 张新胜 张文学 徐存拴 |
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| 作者单位： | [1]河南师范大学生命科学学院，新乡453002 [2]河南省生物工程重点实验室 |
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| 基金项目： | 中法先进技术合作项目(B96-11)，河南省动物学重点学科资助 |
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| 摘 要： | 用免疫组织化学和聚丙烯酰胺凝胶同工酶电泳方法研究了小鼠肾在热休克(46℃,30分钟)恢复期(4h和12h)HSP70的表达和乳酸脱氢酶(LDH)同工酶的变化。结果表明:(1)HSP70主要定位于肾小管上皮细胞胞质中,细胞核内未见表达;(2)HSP70免疫阳性反应在肾髓质较肾皮质强,肾小管较肾小球强;(3)热休克诱导小鼠肾LDH同工酶活性增强。提示:LDH同工酶可能对细胞热耐受性的建立有重要作用。
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| 关 键 词： | 热休克反应 小鼠肾HSP70 乳酸脱氢酶同工酶 表达变化 热休克蛋白70 |
| 修稿时间： | 2001年10月22 |
STUDY ON THE EXPRESSION OF HSP70 AND THE CHANGES OF LDH ISOZYMES OF MOUSE KIDNEY IN HEAT SHOCK RESPONSE |
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| WEI ji Dong ZHANG Shun Li ZHANG Xin Sheng ZHANG Wen Xue XU Cun Shuan.STUDY ON THE EXPRESSION OF HSP70 AND THE CHANGES OF LDH ISOZYMES OF MOUSE KIDNEY IN HEAT SHOCK RESPONSE[J].Chinese Journal of Cell Biology,2002,24(4):242-245. |
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| Authors: | WEI ji Dong ZHANG Shun Li ZHANG Xin Sheng ZHANG Wen Xue XU Cun Shuan |
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| Abstract: | The immunochemical method and PAGE were used to analyze the changes of the expression of HSP70 and lactate dehydrogenase (LDH)isozymes of mouse kidney during the recovery period of heat shock(46t ,30min).The results showed that:(1)the HSP70 positive immunoreactivity was localized in the renal tubule epithelial cytoplasm, while the nucleus was negative; (2)The HSP70 positive im-munoreactivity of the renal medulla was stronger than that of the renal cortex, and the HSP70 positive immunoreactivity of the renal tubule was stronger than that of the renal corpuscle; (3)Heat shock induced increase in the activities of LDH isozymes in kidney. The results suggested that LDH isozymes may be play an important role in the building of heat tolerance of cells. |
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| Keywords: | Heat shock protein 70(HSP70) LDH isozyme Heat shock response Immunohistochetnistry Kidney |
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