Detection of protease activity with a fluorescence-labelled peptide substrate on a TLC plate |
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| Authors: | Uchikoba T Yonezawa H Arima K Kaneda M |
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| Institution: | Department of Chemistry, Faculty of Science, Kagoshima University, Korimoto, Japan. uchik@sci.kagoshima-u.ac.jp |
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| Abstract: | A small amount of peptidase activity could be detected using an amine derivatizing reagent, fluorescein isothiocyanate (FITC), which has been used to produce a fluorogenic peptide. The substrate produced, FITC-peptide, gave a clear spot on a silica gel sheet upon exposure to UV light. The peptidase activity of angiotensin-converting enzyme (ACE), trypsin, chymotrypsin, cucumisin, and that of some plant tissues were detected by using a fluorogenic angiotensin I. This showed that the substrate specificity of proteolytic enzymes can be distinguished from the others by this procedure. |
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