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| 响应面法优化蝙蝠蛾拟青霉发酵培养基配方 | |
| 引用本文： | 巩伯梁,董冰雪,李长杰,张丽萍,李刚. 响应面法优化蝙蝠蛾拟青霉发酵培养基配方[J]. 微生物学免疫学进展, 2011, 39(4): 17-23 |
| 作者姓名： | 巩伯梁 董冰雪 李长杰 张丽萍 李刚 |
| 作者单位： | 1. 深圳科兴生物工程有限公司,广东深圳,518057 2. 南阳师范学院生命科学与技术学院,河南南阳,473061 3. 中山大学基因工程教育部重点实验室,广东广州,510275 |
| 基金项目： | 中山大学青年教师培育项目(编号:111gpy23)资助 |
| 摘 要： | 为了提高虫草无性型蝙蝠蛾拟青霉(Paecilomyces hepiali)菌丝体中的腺苷含量,采用响应面法对其液体发酵培养基配方进行了优化。首先采用Plackett-Burman设计法对影响蝙蝠蛾拟青霉腺苷收率的8个相关因素进行了筛选,从中确定其主要影响因子为马铃薯和蚕蛹粉。然后用最陡爬坡试验逼近上述2个因素的最大响应区域,并通过中心组合设计和响应面分析法,确定了主要影响因子的最佳浓度。优化后的培养基组成为:马铃薯8.25%,蔗糖1%,玉米粉1%,蛋白胨0.5%,蚕蛹粉0.81%,KH2PO40.1%,MgSO4.7H2O 0.1%,(NH4)2 SO40.1%。该配方所产生的蝙蝠蛾拟青霉菌丝体中腺苷含量由优化前的2.15 mg/g(干重)提高到3.44 mg/g(干重)。 |
| 关 键 词： | 蝙蝠蛾拟青霉 Plackett-Burman设计法 中心组合设计 响应面法 腺苷 |
Response surface optimization of the medium components used for cultivation Paecilomyces hepialid |
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| GONG Bo-liang,DONG Bing-xue,LI Chang-jie,et al.. Response surface optimization of the medium components used for cultivation Paecilomyces hepialid[J]. Progress In Microbiology and Immunology, 2011, 39(4): 17-23 | |
| Authors: | GONG Bo-liang DONG Bing-xue LI Chang-jie et al. |
| Affiliation: | GONG Bo-liang1,DONG Bing-xue2,LI Chang-jie3,et al.(1.Shenzhen Kexing Co.Ltd,Shenzhen,Guangdong 518057,China,2.School of Life Science and Technology,Nanyang Normal University,Nanyang,Henan 473061,3.Key Laboratory of gene engineering of the ministry of education,Sun Yat-sen University,Guangzhou,Guangdong 510275,China) |
| Abstract: | The response surface methodology was used to optimize submerged fermentation medium components,in order to improve yeild of mycelial adenosine content from Paecilomyces hepialid.Firstly,The Plackett-Burman Design was used to evaluate the effects of eight variables on adenosine content,and two factors including potato and silkworm powder were found to have significant effect on adenosine content of Paecilomyces hepialid.Then,the steepest ascent method was used to determine their optimal levels to lead the ex... |
| Keywords: | Paecilomyces hepialid Plackett-Burman(PB)Design Central composite design Response surface methodology Adenosine |
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