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Cloning of Sal1, a scytalone dehydratase gene involved in melanin biosynthesis in Cochliobolus heterostrophus |
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| Authors: | Yoshimoto Saitoh Kosuke Izumitsu Atsushi Morita Kiminori Shimizu Chihiro Tanaka |
| Institution: | 1. Sustainability Research Institute, Tottori University of Environmental Studies, Tottori, 689-1111, Japan 2. Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, Chiba, 270-1194, Japan 4. Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan 3. Medical Mycology Research Center, Chiba University, Chiba, 260-8673, Japan |
| Abstract: | A melanin biosynthetic gene, Sal1, in the southern corn leaf blight fungus Cochliobolus heterostrophus, was identified by degenerate and inverse PCR. Structural analysis revealed that the open reading frame is 555 bp in length, interrupted by one intron. Melanin deficiency of the sal1 mutant strain, which accumulates the intermediate scytalone, was successfully complemented by introducing the gene fragment. These results suggest that Sal1 is a scytalone dehydratase gene of C. heterostrophus. |
| Keywords: | Color mutant 1 8-Dihydroxynaphthalene SCD |
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