Inheritance of somatic embryogenesis in red clover (Trifolium pratense L.) |
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Authors: | N L McLean J Nowak |
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Institution: | (1) Department of Plant Science, Nova Scotia Agricultural College, PO Box 550 Truro, Nova Scotia, Canada B2N 5E3 Fax: +1 (902) 897-9762 E-mail: nmclean@cox.nsac.ns.ca, CA |
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Abstract: | Red clover genotypes capable of regenerating plantlets in vitro from non-meristematic tissue-derived callus are rare. Selection
for genotypes capable of somatic embryogenesis identified a clone comprised of a group of plantlets regenerated from a hypocotyl-derived
callus culture on L2-based media and another group of plantlets originating from crown divisions of the epicotyl-derived plant.
The callus-derived plants of this clone were highly regenerative when reintroduced to callus culture, but the epicotyl-derived
plants produced nonregenerative callus cultures. F1, F2 and BC1 populations were evaluated to determine the mode of inheritance of the regeneration trait. Reciprocal crosses did not differ,
indicating a lack of maternal effects. Results were compatible with genetic control of regeneration by two complementary genes.
We propose the genotype Rn1-Rn2- for regenerative plants. Three petiole segment explants were sufficient to evaluate regenerative ability in seedlings. Regenerative
ability was often associated with abnormal leaf morphology in a few to several leaves.
Received: 17 February 1998 / Accepted: 7 April 1998 |
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Keywords: | Red clover Trifolium pratense Somatic embryogenesis Inheritance |
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