A capacity for mixing in capillary wells for microplates |
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Authors: | Jonathan Kok Keung Lye Tuck Wah Ng Adrian Neild Oi Wah Liew |
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Affiliation: | aLaboratory for Optics, Acoustics, and Mechanics, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia;bCardiovascular Biomarkers Laboratory, Cardiovascular Research Institute, 30 Medical Drive 117609 Singapore, Singapore |
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Abstract: | Sample/reagent mixing is important in microplate instrumentation and the approach of mixing prior to dispensation into the wells can be problematic when assays involve a mixture of different proportions of reagents. We demonstrate here with capillaries, a simple and highly effective method that uses air actuation and liquid surface tension to create pendant drops in which the inherent inner circulatory flow within accomplishes mixing. The approach portends the capability to be incorporated into microplates in order to provide a useful feature for assay investigations. |
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