Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation |
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| Authors: | Fujihara Junko Tabuchi Mari Inoue Takayuki Yasuda Toshihiro Fujita Yasuhisa Takeshita Haruo |
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| Institution: | aDepartment of Legal Medicine, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan;bDepartment of Chemistry, Graduate School of Science, Rikkyo University, Toshima, Tokyo 171-8501, Japan;cDivision of Medical Genetics and Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui-shi, Fukui 910-8507, Japan;dInterdisciplinary Faculty Science and Engineering, Shimane University, Matsue-shi, Shimane 690-8504, Japan |
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| Abstract: | Deoxyribonuclease I (DNase I) activity in serum has been shown to be a novel diagnostic marker for the early detection of acute myocardial infarction (AMI). However, the conventional method to measure DNase I activity is time-consuming. In the current study, to develop a rapid assay method for DNase I activity for clinical purposes, a microchip electrophoresis device was used to measure DNase I activity. Because DNase I is an endonuclease that degrades double-stranded DNA endo-nucleolytically to produce oligonucleotides, degradation of the DNA standard caused by DNase I action was detected using microchip electrophoresis. We detected DNase I activity within 10 min. This is the first study to apply microchip electrophoresis for the detection of DNase I activity; furthermore, it seems plausible that reduction of analysis time for DNase I activity could make this novel assay method using microchip electrophoresis applicable in clinical use. |
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