Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis |
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| Authors: | Yang Bo Weyers Amanda Baik Jong Youn Sterner Eric Sharfstein Susan Mousa Shaker A Zhang Fuming Dordick Jonathan S Linhardt Robert J |
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| Institution: | aDepartment of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA;bCollege of Nanoscale Science and Engineering, University at Albany, Albany, NY 12203, USA;cDepartment of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA;dPharmaceutical Research Institute at Albany College of Pharmacy, Albany, NY 12208, USA;eDepartment of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA;fDepartment of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA |
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| Abstract: | A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities. |
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| Keywords: | Heparin Heparan sulfate Heparin lyase Disaccharide analysis Biological samples Ultra-performance liquid chromatography Mass spectrometry |
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