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Equilibration of H labeling between body water and free amino acids: Enabling studies of proteome synthesis |
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| Authors: | Kithsiri Herath Gowi Bhat Paul L Miller Sheng-Ping Wang Alison Kulick Genevieve Andrews-Kelly Christopher Johnson Rory J Rohm Michael E Lassman Stephen F Previs Douglas G Johns Brian K Hubbard Thomas P Roddy |
| Institution: | aAtherosclerosis, Merck Research Laboratories, Rahway, NJ 07065, USA;bLaboratory Animal Resources, Merck Research Laboratories, Rahway, NJ 07065, USA |
| Abstract: | Protein synthesis can be estimated by measuring the incorporation of a labeled amino acid into a proteolytic peptide. Although prelabeled amino acids are typically administered, recent studies have tested 2H2O; the assumption is that there is rapid equilibration of 2H (in body water) with the carbon-bound hydrogens of amino acids before those amino acids are incorporated into a protein(s). We have determined the temporal changes in 2H labeling of body water and amino acids which should build confidence in 2H2O-based studies of protein synthesis when one aims to measure the 2H labeling of proteolytic peptides. |
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