Facile synthesis of metal-chelating magnetic nanoparticles by exploiting organophosphorus coupling |
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Authors: | Kun Yang Wei Wen Su |
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Affiliation: | Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI 96822, USA |
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Abstract: | A new method is described for facile synthesis of metal-chelating magnetic nanoparticles by simply mixing iron oxide nanoparticles with a bifunctional organophosphorus compound, N-(phosphonomethyl)iminodiacetic acid (PM–IDA), in aqueous solution. On charging with nickel ions, the PM–IDA functionalized iron oxide nanoparticles exhibited high His-tag protein binding capacity (0.21 and 0.58 mg/mg for His-tagged green fluorescent protein and chloramphenicol acetyltransferase, respectively) and were successfully used to purify these proteins from bacterial cell extracts to high purity in a single step. Although other synthetic schemes for metal-chelating magnetic nanoparticles have been reported, the method described here is markedly simpler and involves only low-cost reagents. |
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