Soluble chemokine receptor CXCR4 is present in human sera |
| |
| Authors: | Malvoisin Etienne Livrozet Jean-Michel Makloufi Djamila Vincent Nadine |
| |
| Affiliation: | aFédération de Biochimie, Hôpital Edouard Herriot, CHU-Lyon 69437, France;bDépartement de Transplantation et Immunologie Clinique, Hôpital Edouard Herriot, CHU-Lyon 69437, France;cGroupe Immunité des Muqueuses et Agents Pathogènes, Faculté de Médecine, Saint-Etienne 42023, France |
| |
| Abstract: | A soluble form of the chemokine receptor CXCR4 was detected in human sera by isoelectric focusing and Western blotting. Sera of patients and normal subjects were analyzed using a panel of specific antibodies. Compared with controls, high levels of serum CXCR4 were found in patients with inflammatory bowel diseases. Serum CXCR4 levels in the majority of HIV patients were similar to those in healthy controls. A sensitive polyclonal antibody was developed in rabbit immunized with a maltose binding protein (MBP) construct expressing the full-length CXCR4. Using anti-MBPCXCR4 antibody, the level of CXCR4 in sera of a majority of patients with fibrosis was very low. The potential of serum CXCR4 as a new diagnostic biomarker warrants further investigation. |
| |
| Keywords: | CXCR4 Isoelectric focusing HIV-1 |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
