Multiple target loci assembly sequencing (mTAS) |
| |
| Authors: | Han Hyojun Yoon Jung-Ki Cho Byoung Chul Kim Hwangbeom Bang Duhee |
| |
| Affiliation: | aDepartment of Chemistry, Yonsei University, Shinchon 134, Seoul 120-749, Republic of Korea;bCollege of Medicine, Seoul National University, Seoul 110-799, Republic of Korea;cDepartment of Internal Medicine, College of Medicine, Yonsei University, 250 Seongsanno, Seodaemun-gu, Seoul 120-752, Republic of Korea |
| |
| Abstract: | Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
