Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer |
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| Authors: | Song Kyung-Mi Cho Minseon Jo Hunho Min Kyoungin Jeon Sung Ho Kim Taisun Han Min Su Ku Ja Kang Ban Changill |
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| Institution: | aDepartment of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk 790-784, South Korea;bDepartment of Mechanical Engineering and Department of Materials, University of California, Santa Barbara, Santa Barbara, CA 93106, USA;cDepartment of Life Science, Hallym University, Chuncheon-si, Kangwon-do 200-702, South Korea;dDepartment of Chemistry, Hallym University, Chuncheon-si, Kangwon-do 200-702, South Korea;eDepartment of Chemistry, Chuang-Ang University, Seoul 156-765, South Korea |
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| Abstract: | A selective kanamycin-binding single-strand DNA (ssDNA) aptamer (TGGGGGTTGAGGCTAAGCCGA) was discovered through in vitro selection using affinity chromatography with kanamycin-immobilized sepharose beads. The selected aptamer has a high affinity for kanamycin and also for kanamycin derivatives such as kanamycin B and tobramycin. The dissociation constants (Kd kanamycin] = 78.8 nM, Kd kanamycin B] = 84.5 nM, and Kd tobramycin] = 103 nM) of the new aptamer were determined by fluorescence intensity analysis using 5′-fluorescein amidite (FAM) modification. Using this aptamer, kanamycin was detected down to 25 nM by the gold nanoparticle-based colorimetric method. Because the designed colorimetric method is simple, easy, and visible to the naked eye, it has advantages that make it useful for the detection of kanamycin. Furthermore, the selected new aptamer has many potential applications as a bioprobe for the detection of kanamycin, kanamycin B, and tobramycin in pharmaceutical preparations and food products. |
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| Keywords: | ssDNA aptamer SELEX Colorimetry Gold nanoparticle Kanamycin |
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