Temperature-dependent selective purification of plasmid DNA using magnetic nanoparticles in an RNase-free process |
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| Authors: | Shan Zhi Li Chenglei Zhang Xu Oakes Ken D Servos Mark R Wu Qi Chen Hui Wang Xianxiang Huang Qianming Zhou Yi Yang Wanshen |
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| Affiliation: | aInstitute of Chemical Engineering, National Taipei University of Technology, Taipei 10608, Taiwan;bSchool of Medicine, Taipei Medical University, Taipei 11031, Taiwan;cDivision of Chemical Biology, Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan |
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| Abstract: | The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments. |
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