小麦试管苗的管理及染色体加倍与移栽的研究

STUDIES ON CHROMOSOME DOUBLING, TRANSPLANTING AND MANAGEMENT OF TUBE SHOOTS IN WHEAT

N_6 agar medium with 0.2mg IAA, 40mg Ad and 20g sucrose perliter has good effect on promoting sturdy growth and development oftube shoots and their roots, after 40 days, per tube shoot can develop2.65 new roots. The oversummer sturdy tube shoots can be obtainedboth in controlling the main stems with 6-7 leaves and in the youngspike differentiation from the elongating to the single-rowed periodthrough regulating light. Befoer transplanting. the tube shoots soaked into the simple nutrient solution for 6-7 days, thus, per tube shootcan produce 1.16 new roots and the root-producing rate of the rootlesstube shoots is 41.67%. After the shoots are cultured in the nutrientpaper pots for 20-25 days, they are transplanted into the field withnutrient paper pots, the survived rate is 96.88%. The chromosomedoubling of tube shoots can be carried out in winter and spring, thebest time is from Jan. 15 to Feb. 15. The roots of tube shoots soakedinto colchicine solution containing nutrient (the simple nutrient solu-tion +0.05% of colchicine+1.5% of DMSO, with pH7), the survived rateand the doubling rate of plants are 88.10% and 52.70% respectively.