猪耳皮肤成纤维细胞的培养

本研究以猪耳皮组织为材料，采用胰蛋白酶冷热处理结合法成功地分离和培养了猪耳皮肤成纤维细胞。此方法的细胞存活率相对于胰蛋白酶热处理法较高。传代细胞与原代细胞的形态和生长速度均相似。传代细胞未检测到凋亡现象。细胞已传至15代以上，染色体倍性正常，说明我们所建立的胰蛋白酶冷热处理结合法可以快速有效的分离和培养猪耳皮肤成纤维细胞，并且能稳定的进行传代培养。

CULTURE OF PORCINE EAR SKIN FIBROBLASTS

In this research, the porcine ear skin fibroblasts were successfully dissociated and cultured by using the porcine ear tissue as materials. The research adopted a kind of method combining trypsin cold treatment with trypsin heat treatment. The rate of living cells in this method was much higher than that of trypsin heat treatment. The passage cells were similar to the primary culture in shape and on the growth rate. The apoptosis in the passage cells was not been detected. Cells were subcultured to fifteenth passage. The chromosomes of passage 12 and passage 15 cells were analysed,and the results showed that they contained the euploid chromosome number of n=38. Thus it was illustrated that the porcine ear skin fibroblasts could quickly and efficiently be dissociated and cultured, and the subculturing was stable by using this kind of method combining trypsin cold treatment with trypsin heat treatment.