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Kinetics Characterization of Taurocholic Transport in Lactobacillus reuteri
Authors:María Pía Taranto  Christian Magni  Diego de Mendoza  Graciela Font de Valdez
Affiliation:(1) Centro de Referencia para Lactobacilos (CERELA, CONICET), Chacabuco 145, 4000- S. M. de Tucumán, Argentina, AR;(2) Programa Multidisciplinario de Biología Experimental (PROMUBIE, CONICET) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina, AR
Abstract:Taurocholic acid transport in Lactobacillus reuteri CRL 1098 was determined. The bile acid is incorporated inside the cells by an active and saturable transport showing a typical kinetics of Michaelis-Menten with values of K m and V max of 0.35 mm and 20 mm, respectively. Received: 30 May 2000/Accepted: 5 July 2000
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