Improving the baculovirus expression vector system with vankyrin‐enhanced technology |
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Authors: | Kendra H. Steele Barbara J. Stone Kathleen M. Franklin Angelika Fath‐Goodin Xiufeng Zhang Haobo Jiang Bruce A. Webb Christoph Geisler |
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Affiliation: | 1. ParaTechs Corporation, Lexington, KY;2. Dept. of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma;3. ParaTechs Corporation, Lexington Kentucky, Department of Entomology, University of Kentucky, Lexington, KT;4. GlycoBac LLC, Laramie, Wyoming |
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Abstract: | The baculovirus expression vector system (BEVS) is a widely used platform for the production of recombinant eukaryotic proteins. However, the BEVS has limitations in comparison to other higher eukaryotic expression systems. First, the insect cell lines used in the BEVS cannot produce glycoproteins with complex‐type N‐glycosylation patterns. Second, protein production is limited as cells die and lyse in response to baculovirus infection. To delay cell death and lysis, we transformed several insect cell lines with an expression plasmid harboring a vankyrin gene (P‐vank‐1), which encodes an anti‐apoptotic protein. Specifically, we transformed Sf9 cells, Trichoplusia ni High FiveTM cells, and SfSWT‐4 cells, which can produce glycoproteins with complex‐type N‐glycosylation patterns. The latter was included with the aim to increase production of glycoproteins with complex N‐glycans, thereby overcoming the two aforementioned limitations of the BEVS. To further increase vankyrin expression levels and further delay cell death, we also modified baculovirus vectors with the P‐vank‐1 gene. We found that cell lysis was delayed and recombinant glycoprotein yield increased when SfSWT‐4 cells were infected with a vankyrin‐encoding baculovirus. A synergistic effect in elevated levels of recombinant protein production was observed when vankyrin‐expressing cells were combined with a vankyrin‐encoding baculovirus. These effects were observed with various model proteins including medically relevant therapeutic proteins. In summary, we found that cell lysis could be delayed and recombinant protein yields could be increased by using cell lines constitutively expressing vankyrin or vankyrin‐encoding baculovirus vectors. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1496–1507, 2017 |
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Keywords: | vankyrin baculovirus glycosylation difficult to express proteins SfSWT |
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