Diversity Profile and Dynamics of Peptaibols Produced by Green Mould Trichoderma Species in Interactions with Their Hosts Agaricus bisporus and Pleurotus ostreatus |
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| Authors: | Tamás Marik Péter Urbán Chetna Tyagi András Szekeres Balázs Leitgeb Máté Vágvölgyi László Manczinger Irina S. Druzhinina Csaba Vágvölgyi László Kredics |
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| Affiliation: | 1. Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary;2. Department of General and Environmental Microbiology, Faculty of Sciences, and Szentágothai Research Center, University of Pécs, Pécs, Hungary;3. Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary;4. Institute of Pharmacognosy, University of Szeged, Szeged, Hungary;5. Research Area Biochemical Technology, Institute of Chemical and Biological Engineering, TU Wien, Vienna, Austria |
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| Abstract: | Certain Trichoderma species are causing serious losses in mushroom production worldwide. Trichoderma aggressivum and Trichoderma pleuroti are among the major causal agents of the green mould diseases affecting Agaricus bisporus and Pleurotus ostreatus, respectively. The genus Trichoderma is well‐known for the production of bioactive secondary metabolites, including peptaibols, which are short, linear peptides containing unusual amino acid residues and being synthesised via non‐ribosomal peptide synthetases (NRPSs). The aim of this study was to get more insight into the peptaibol production of T. aggressivum and T. pleuroti. HPLC/MS‐based methods revealed the production of peptaibols closely related to hypomurocins B by T. aggressivum, while tripleurins representing a new group of 18‐residue peptaibols were identified in T. pleuroti. Putative NRPS genes enabling the biosynthesis of the detected peptaibols could be found in the genomes of both Trichoderma species. In vitro experiments revealed that peptaibols are potential growth inhibitors of mushroom mycelia, and that the host mushrooms may have an influence on the peptaibol profiles of green mould agents. |
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| Keywords: | Trichoderma green mould Cultivated mushrooms Peptaibol Liquid chromatography Mass spectrometry |
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