| 快速连续测定乙肝病毒DNA酶解大片段的顺序 |
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| 引用本文： | 琦祖和,阎珺,熊伟军.快速连续测定乙肝病毒DNA酶解大片段的顺序[J].中国生物化学与分子生物学报,1988,4(1):42-47. |
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| 作者姓名： | 琦祖和 阎珺 熊伟军 |
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| 作者单位： | 中国协和医科大学基础部生化室,中国医学科学院基础医学所生化室 北京,北京 |
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| 摘 要： | 用“剪切重组”术及内引物法测定了克隆在M_(13)mp_8载体中的HBVDNA 1.3Kb片段的完整顺序,简化了操作过程,为连续测定大段DNA的顺序提供了简单、快速、有效的途径。
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| 关 键 词： | 乙肝病毒DNA “剪切重组” 内引物 定点克隆 |
| 收稿时间： | 1988-02-20 |
RAPID AND SYSTEMATIC DETERMINATION OF THE SEQUENCE OF HBV adr NC-1 DNA 1.3 kb FRAGMENT |
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| Qi,Zu-he Yan,Jun Xung,Wei-jun.RAPID AND SYSTEMATIC DETERMINATION OF THE SEQUENCE OF HBV adr NC-1 DNA 1.3 kb FRAGMENT[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(1):42-47. |
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| Authors: | Qi Zu-he Yan Jun Xung Wei-jun |
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| Institution: | (Dept.of Biochem., Faculty of Basic Medicine, Peking Union Medical College)(Dept.of Biochem, Inst.of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing |
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| Abstract: | In sequencing the HBV DNA 1.3 kb fragment inserted in vector M13mp8 we developed a "cutting religation" procedure and combined using synthesized internal primers. These procedures simplified the operations and made the determination of large DNA fragment easier and more rapid. |
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| Keywords: | HBV DNA Cutting religation Intermal primers Map cloning |
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