| 利用荧光分光光度计定量分析GFP基因的表达水平 |
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| 引用本文： | 苟吉庆,李敏,张锐,郭三堆.利用荧光分光光度计定量分析GFP基因的表达水平[J].生物技术通报,2001,58(5):27-29. |
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| 作者姓名： | 苟吉庆 李敏 张锐 郭三堆 |
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| 作者单位： | 中国农业科学院生物技术研究所 |
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| 摘 要： | 以3种表达水分高低不一的绿色组织特异性启动子驱动绿色光蛋白（green fluorescent protein,GFP）基因转化烟草植株，设计了一种利用荧光分光光度计对组织中GFP的表达水平进行定量分析的新方法，利用该方法对获得的102株转基因烟草中不同部位叶片中的GFP表达水平进行了定量分析.其结果与荧光显微镜观察结果高度一致，从而证实利用这种新方法对GFP基因进行定量分析是可行的。
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| 关 键 词： | 绿色组织特异性启动子 绿色荧光蛋白 荧光分光光度计 基因表达水平 定量分析 |
Quantitative Analysis of GFP Expression Level With Fluorescent Spectrophotometer |
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| Gou Jiqing,Li Min Zhang Rui Guo Sandui.Quantitative Analysis of GFP Expression Level With Fluorescent Spectrophotometer[J].Biotechnology Bulletin,2001,58(5):27-29. |
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| Authors: | Gou Jiqing Li Min Zhang Rui Guo Sandui |
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| Abstract: | Green fluorescent protein gene was placed under three different green tissue-specific promoters and transferred into tobacco plants. A new kind of methods was designed to quantitatively analyze GFP expression level in transgenic plants use fluorescent spectrophotometer. The results were highly conformed to those gained by fluorescent microscopy. This result implies that the new method is capable for quantitative analysis of the GFP expression level. |
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| Keywords: | green tissue-specific promoters Green fluorescent protein fluorescent spectrophotometer gene expressing level quantitative analysis |
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