Kunitz-type trypsin inhibitor with high stability from Spinacia oleracea L. seeds |
| |
Authors: | Zhuang Kang Jia-hong Jiang Dong Wang Ke Liu Lin-fang Du |
| |
Affiliation: | (1) Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, Sichuan University, Chengdu, 610064, P. R. China;(2) Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, 610064, China |
| |
Abstract: | The trypsin inhibitor SOTI was isolated from Spinacia oleracea L. seeds through ammonium sulfate precipitation, Sepharose 4B-trypsin affinity chromatography, and Sephadex G-75 chromatography. This typical Kunitz inhibitor showed remarkable stability to heat, pH, and denaturant. It retained 80% of its activity against trypsin after boiling for 20 min, and more than 90% activity when treated with 6 M guanidine hydrochloride. The formation of stable SOTI-trypsin complex (K i = 2.3·10−6 M) is consistent with significant inhibitory activity of SOTI against trypsin-like proteinases present in the larval midgut of Pieris rapae. Sequences of SOTI fragments showed homology with other inhibitors. Published in Russian in Biokhimiya, 2009, Vol. 74, No. 1, pp. 131–140. |
| |
Keywords: | Spinacia oleracea L. trypsin inhibitor protein stability Kunitz family Pieris rapae |
本文献已被 SpringerLink 等数据库收录! |
|