| 斑马鱼新基因KLP的筛选、克隆及初步分析 |
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| 引用本文： | 李培,杨寒朔,魏于全,邓洪新. 斑马鱼新基因KLP的筛选、克隆及初步分析[J]. 四川动物, 2007, 26(4): 796-799 |
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| 作者姓名： | 李培 杨寒朔 魏于全 邓洪新 |
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| 作者单位： | 四川大学生命科学学院,成都610064;四川大学华西医院生物治疗国家重点实验室;四川大学华西医院生物治疗国家重点实验室 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;四川大学学科建设基金 |
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| 摘 要： | 通过重组cDNA表达文库的血清学分析(SEREX)获得来自斑马鱼的cDNA序列,提取斑马鱼总RNA通过RT-PCR得到该基因全长共5171 bp编码1574个氨基酸,运用生物信息学研究工具进行分析显示该基因为一新基因,命名为KLP,在斑马鱼胚胎各个发育时期用RT-PCR检测该基因的表达情况发现:在斑马鱼早期胚胎发育的几个重要时期该基因都有高表达。该基因含有8个锌指结构,其中3个KAISO蛋白特征性的C2H2锌指结构,且此区域在多个物种内高度保守,提示其在胚胎发育早期过程中具有非常重要的作用。
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| 关 键 词： | 斑马鱼 新基因 基因克隆 SEREX |
| 文章编号： | 1000-7083（2007）04-0796-04 |
| 修稿时间： | 2007-04-13 |
Clone and Preliminary Characterization of a Novel KLP Gene in Zebrafish |
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| LI Pei,YANG Han-shuo,WEI Yu-quan,DENG Hong-xin. Clone and Preliminary Characterization of a Novel KLP Gene in Zebrafish[J]. Sichuan Journal of Zoology, 2007, 26(4): 796-799 |
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| Authors: | LI Pei YANG Han-shuo WEI Yu-quan DENG Hong-xin |
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| Affiliation: | 1. College of Life Sciences, Sichuan University, Chengdu 610064; 2. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University |
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| Abstract: | we found a cDNA sequencer from the zebrafish by serological analysis of recombinant cDNA expression library(SEREX).The bioinformatic analysis results show that the novel gene has 5170 bp at all and codes a 1574 amino acid protein,which contains 8 zinc fingers.Among the 8 zinc fingers,the 3 N-terminal zinc fingers resemble that of KAISO,so we named this novel gene as KLP(KAISO like protein),and this district is high conservative in several species.We analyzed the expressoin of KLP during the zebrafish development by RT-PCR.The results show this gene has important function in the earlier period of zebrafish embryo development. |
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| Keywords: | SEREX |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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