Enumeration of airborne bacteria and fungi using solid phase cytometry |
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Authors: | Vanhee Lies M E Nelis Hans J Coenye Tom |
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Institution: | Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, Ghent, Belgium. |
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Abstract: | Conventional methods for the enumeration of airborne micro-organisms are inaccurate and time-consuming, hence the interest in novel approaches is increasing. In the present study, the use of solid phase cytometry (SPC) was evaluated for the enumeration of airborne micro-organisms. A 4 h SPC procedure based on viability staining was applied to samples from 50 locations and compared with an optimised culture-based method. Plate counts after air sampling were repeatable but strongly dependent on sampling volume. Samples with low or high microbial load were difficult to analyse using the culture-based method, unlike with SPC. Results show that SPC can be considered superior to the culture-based method because of its much higher dynamic range, its speed and its ability to enumerate not only culturable but all viable micro-organisms. |
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