Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L. |
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| Authors: | X Barandiaran A Di Pietro J Martín |
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| Institution: | (1) Departamento de Microbiología, Facultad de Ciencias, Universidad de Córdoba, Córdoba, Spain Fax no.: +34-57-21 86 06 e-mail: milmasaj@uco.es, ES;(2) Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba, Córdoba, Spain, ES |
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| Abstract: | The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity
preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mm solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased
the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results.
Received: 16 May 1997 / Revision received: 26 August 1997 / Accepted: 25 September 1997 |
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| Keywords: | Allium sativum Transformation Particle bombardment Nuclease Aurintricarboxylic acid |
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