Enzyme inhibition by p-cresol and lacticacid in lipase-mediated synthesis of p-cresyl acetate and stearoyl lactic acid: a kinetic study |
| |
Authors: | Kiran KR Divakar S |
| |
Institution: | (1) Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570 013, India |
| |
Abstract: | A detailed kinetic study was carried out to investigate the porcine pancreatic lipase-catalysed esterification reactions of p-cresol–acetic acid and lactic acid–stearic acid. The kinetic data were in agreement with a Ping Pong Bi–Bi mechanism being followed by the enzyme, where inhibition is indicated in the presence of p-cresol and lactic acid in the respective reactions. Mathematical analyses of experimentally observed initial rates yielded various kinetic parameters, K
m(p-cresol) = 0.1, K
m(acetic acid) = 0.54, K
m(lactic acid) = 0.059 M, K
m(stearic acid) = 0.04 M, V
max(p-cresol–acetic acid) = 13.2(h–1), V
max(lactic acid–stearic acid) = 0.00163 M/h, K
i(p-cresol) = 0.59 and K
i(lactic acid) = 0.079 M. The K
m and K
i values of p-cresol and lactic acid observed in the respective reactions showed both the competitive nature of binding between the substrates p-cresol and acetic acid on the one hand and lactic acid and stearic acid on the other and the inhibitory nature of p-cresol and lactic acid. |
| |
Keywords: | Enzyme kinetics inhibition kinetic parameters p-cresyl acetate Ping-Pong Bi– Bi mechanism porcine pancreatic lipase stearoyl lactic acid |
本文献已被 SpringerLink 等数据库收录! |
|