Purification and characterization of two isoforms from Candida rugosa lipase B |
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| Authors: | Cristina López Nelson P Guerra M Luisa Rúa |
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| Institution: | (1) Area de Bioquímica e Bioloxía Molecular, Facultade de Ciencias de Ourense, Universidade de Vigo, 32004 Ourense, Spain |
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| Abstract: | Two isoforms of Candida rugosalipase B (LB1 and LB2) were purified by anionic exchange chromatography. The lipases had the same N-terminal sequence, carbohydrate content and pH and thermal stability but different pIs and significant differences in their activities against different p-nitrophenol esters and triacylglycerides. |
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| Keywords: | Candida rugosa characterization isoforms lipase B purification |
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