Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L. |
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Authors: | Angela Carra Maria Beatrice Del Signore Francesco Sottile Ada Ricci Francesco Carimi |
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Institution: | (1) Istituto di Genetica Vegetale del Consiglio Nazionale delle Ricerche UOS di Palermo, Corso Calatafimi 414, 90129 Palermo, Italy;(2) EnBioTech s.r.l., Via Aquileia 34/B, 90144 Palermo, Italy;(3) Dipartimento DEMETRA dell’Universit? di Palermo, Viale delle Scienze 11, 90128 Palermo, Italy;(4) Dipartimento di Biologia Evolutiva e Funzionale, Universit? di Parma, Viale G.P. Usberti 11/A, 43100 Parma, Italy; |
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Abstract: | A protocol for in vitro multiplication of caper (Capparis spinosa L. subsp. rupestris) from nodal segments collected from mature plants was developed. For shoot multiplication, one auxin (indol-3-butyric acid,
IBA) and cytokinins of two different classes were used: the N6-substituted adenine derivatives 6-benzylamino purine (BAP),
and the two synthetic phenylurea derivatives N-phenyl-N′-benzothiazol-6-ylurea (PBU) and N-phenyl-N′-(1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ). Maximum shoot production was achieved from explants cultured with the adeninic
cytokinin BAP (4 μM) and the auxin IBA (0.5 μM). New shoots longer than 1 cm were used for rooting. To induce root formation,
three auxins indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 3-Indoleacetic acid (IAA)] and two synthetic
phenylurea derivatives N,N-bis-(2,3-methylenedioxyphenyl)urea (2,3-MDPU) and N,N-bis-(3,4-methylenedioxyphenyl)urea (3,4-MDPU)] were used. All rooting compounds tested stimulated the formation of roots.
However, the best result in terms of a high percentage of rooted shoots having a well-developed root system with many lateral
roots was achieved with the synthetic phenylurea 2,3-MDPU (1 μM) with 93.7% of well rooted plantlets. About 80% of rooted
plantlets were successfully acclimatized and transferred to the greenhouse. |
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