Selection of optimal reference genes for expression analysis in the entomopathogenic fungus <Emphasis Type="Italic">Beauveria bassiana</Emphasis> during development,under changing nutrient conditions,and after exposure to abiotic stresses |
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Authors: | Yong-Hong?Zhou Yong-Jun?Zhang Zhi-Bing?Luo Yan-Hua?Fan Gui-Rong?Tang Li-Ji?Liu Email author" target="_blank">Yan?PeiEmail author |
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Institution: | (1) Biotechnology Research Center, Southwest University, 215 Tiansheng Road, Beibei, 400716, Chongqing, People’s Republic of China; |
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Abstract: | The selection of suitable reference genes is crucial for accurate quantification of gene expression. To identify suitable
reference genes in Beauveria bassiana, the expression of 14 candidates (18S, 28S, β-Tub, GAPD, γ-Act, TEF, HGPT, His3, His2A, TBP, CypA, CypB, PP1, and CrzA) was measured by quantitative polymerase chain reaction at different development stages and under various nutritional and
stress conditions. Expression stability, as evaluated by the geNorm and NormFinder programs, revealed that His2A/γ-Act/CrzA was the most stably expressed set of genes throughout development, while 28S/PP1/CypA and His2A/γ-Act/CypA were the most stably expressed gene sets under a variety of nutritional and stress conditions, respectively. Overall, the
most stably expressed genes under all conditions examined were PP1, γ-Act, and CypA. |
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