Cell adsorption control by culture conditions |
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Authors: | Jochen Büchs Nava Mozes Christian Wandrey Paul G Rouxhet |
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Institution: | (1) Institute of Biotechnology, KFA Jülich, Postbox 1913, D-5170 Jülich, Federal Republic of Germany;(2) Unité de Chimie des Interfaces, Université Catholique de Louvain, Place Croix du Sud 1, B-1348, Louvain-la-Neuve, Belgium |
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Abstract: | Summary Phosphate supply to growingCorynebacterium glutamicum (i.e. their phosphorus content) was found to have a distinct influence on cell wall properties, resulting in a variation of flocculation and adsorption behaviour of the bacteria. Cells saturated with phosphate are hydrophobic and show a high tendency to flocculate and to adsorb on treated glass surfaces due to hydrophobic interactions. Phosphate depletion of the cells leads to a more hydrophilic surface character and a comparatively low ability to flocculate and to adhere. The net surface charge ofC. glutamicum is not much affected by their phosphorus content. As phosphate depletion (down to a phosphorus content of about 20% of the saturation value) was shown to have no influence on growth rate and on specific 1-leucine productivity, the inducible variation of the surface properties ofC. glutamicum can be exploited to control a continuous reactor with adsorbed cells. |
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