Quantitative evaluation of serum IgG subclass levels by an immunoenzyme method |
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Authors: | P Lambin M Dubarry C Charron N Herance E Defendini |
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Institution: | Laboratoire d'Immunochimie, Institut National de Transfusion Sanguine, Paris, France. |
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Abstract: | We developed a enzyme linked immunosorbent assay (ELISA) for measuring IgG subclasses concentration in serum. For this we used monoclonal antibodies. The specificity of these antibodies was evaluated with a panel of myeloma proteins belonging to the 4 IgG subclasses. The ELISA was sensitive (allowing the detection of subclasses at ng level) and accurate (inter-assay coefficient of variation of 14%). Using the WHO serum 67/97 as reference, we determined the concentration of IgG subclasses in a pool of sera. In addition concentrations were measured in 69 healthy adults to study the distribution of each IgG subclass. A good correlation (r = 0.78) was obtained between the sum of the subclasses measured by ELISA and total IgG measured by immunonephelometry. |
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